Arbeitsgruppe PD Dr. Reiner Vogel

Biophysik, Institut für Molekulare Medizin

Universität Freiburg



Fried SDE, Lewis JW, Szundi I, Martinez-Mayorga M, Mahalingam M, Vogel R, Kliger DS, Brown MF

Membrane Curvature Revisited-the Archetype of Rhodopsin Studied by Time-Resolved Electronic Spectroscopy

Biophys J 120:440      PubMed       URL




Pope A, Sanchez-Reyes OB, South K, Zaitseva E, Ziliox M, Vogel R, Reeves PJ, Smith SO

A conserved proline hinge mediates helix dynamics and activation of rhodopsin

Structure 28:1        PubMed       URL





Kimata N, Pope A, Eilers M, Opefi CA, Ziliox M, Hirshfeld A, Zaitseva E, Vogel R, Sheves M, Reeves PJ, Smith SO

Retinal orientation and interactions in rhodopsin reveal a two-stage trigger mechanism for activation

Nature Communications 7: 12683        PubMed       URL





Tarttelin EE, Fransen MP, Edwards PC, Hankins MW, Schertler GFX, Vogel R, Lucas RJ, Bellingham J

Adaptation of pineal expressed teleost exo-rod opsin  to non-image forming photoreception through enhanced Meta II decay

Cell Mol Life Sci 68: 3713-3723       PubMed       URL





Ye S, Zaitseva E, Caltabiano G, Schertler GFX, Sakmar TP, Deupi X, Vogel R

Tracking G protein-coupled receptor activation using genetically encoded infrared probes

Nature 464:1386-1389      PubMed      URL


Zaitseva E, Brown MF, Vogel R

Sequential rearrangement of interhelical networks for rhodopsin activation in membranes: the Meta IIa conformational substate

J Am Chem Soc 132:4815-4821      PubMed      URL


Zaitseva E, Saavedra M, Banerjee S, Sakmar TP, Vogel R

Surface-enhanced FTIR difference spectroscopy on a membrane protein monolayer using soluble lipoprotein particles as carriers

Biophys J 99:2327-2335    PubMed      URL


Goncalves J, South K, Ahuja S, Zaitseva E, Opefi C, Eilers M, Vogel R, Reeves P, Smith SO

Highly conserved tyrosine stabilizes the active state of rhodopsin

Proc. Natl. Acad. Sci. USA 107:19861-19866      PubMed      URL






Ye S, Huber T, Vogel R, Sakmar TP

FTIR analysis of GPCR activation using azido probes

Nature Chem Biol 5:397-399  PubMed   URL


Lüdeke S, Mahalingam M, Vogel R

Rhodopsin activation switches in a native membrane environment

Photochem Photobiol 85:437-441  PubMed  URL


Schuy S, Schäfer E, Yoder NC, Kumar K, Vogel R, Janshoff A

Lipopeptides derived from HIV and SIV mimicking the prehairpin intermediate of gp41 on solid supported lipid bilayers

J Struct Biol  168:125-136     PubMed  URL


Schuy S, Schäfer E, Yoder NC, Hobe S, Kumar K, Vogel R, Janshoff A

Coiled-coil lipopeptides mimicking the prehairpin intermediate of glycoprotein gp41

Angew Chem Int Ed 48: 751-754     PubMed   URL





Mahalingam M, Martinez-Mayorga K, Brown MF, Vogel R

Two protonation switches control rhodopsin activation in membranes

Proc. Natl. Acad. Sci. USA 105: 17795-17800   PubMed  URL


Jiang X, Zaitseva E, Schmidt M, Engelhard M, Siebert F, Schlesinger R, Ataka K, Vogel R, Heberle J

Resolving voltage-dependent structural changes of a membrane photoreceptor

Proc. Natl. Acad. Sci. USA 105:12113-12117  PubMed  URL


Schuy S, Faiss S, Yoder NC, Kalsani V, Kumar K, Janshoff A, Vogel R

Structure and thermotropic phase behavior of fluorinated phospholipid bilayers: A combined attenuated total reflection IR spectroscopy and imaging ellipsometry study

J Phys Chem B 112: 8250-8256  PubMed  URL


Standfuss J, Zaitseva E, Mahalingam M, Vogel R

Structural impact of the E113Q counterion mutation on the activation and deactivation pathways of the G protein-coupled receptor rhodopsin

J Mol Biol 380: 145-157   PubMed   URL 


Vogel R, Mahalingam M, Lüdeke S, Huber T, Siebert F, Sakmar TP

Functional role of the “ionic lock” – an interhelical hydrogen bond network in family A heptahelical receptors

J Mol Biol 380: 648-655  PubMed URL 





Bartl F, Vogel R

Structural and functional properties of Metarhodopsin III: Recent spectroscopic studies on deactivation pathways of rhodopsin

Phys Chem Chem Phys 9: 1648 - 1658      PubMed    URL


Vogel R, Martell S, Mahalingam M, Engelhard M, Siebert F

Interaction of a G protein-coupled receptor with a G protein-derived peptide induces structural changes in both peptide and receptor:

A Fourier-transform infrared study using isotopically labeled peptides

J Mol Biol 366: 1580-1588   PubMed   URL


Vogel R, Sakmar TP, Sheves M, Siebert F

Coupling of protonation switches during rhodopsin activation

Photochem Photobiol 83: 286-292   PubMed   URL


Yoder NC, Kalsani V, Schuy S, Vogel R, Janshoff A, Kumar K

Nanoscale patterning in mixed fluorocarbon/hydrocarbon phospholipid bilayers

J Am Chem Soc 129: 9037 - 9043     PubMed    URL





Mahalingam M, Vogel R

The all-trans 15-syn retinal chromophore of Metarhodopsin III is a partial agonist and not an inverse agonist

Biochemistry 45: 15624-15632     PubMed   URL 


Vogel R, Siebert F, Yan ECY, Sakmar TP, Hirshfeld A, Sheves M 

Modulating rhodopsin receptor activation by altering the pKa of the retinal Schiff base

J Am Chem Soc 128: 10503-10512   PubMed   URL


Lüdeke S, Lorenz-Fonfria V, Siebert F, Vogel R

Time-resolved rapid-scan FTIR difference spectroscopy on a non-cyclic photosystem: rhodopsin photointermediates from Lumi to Meta II

Biopolymers 83: 159-169    PubMed  URL


Vogel R, Lüdeke S, Siebert F, Sakmar TP, Hirshfeld A, Sheves M

Agonists and partial agonists of rhodopsin: retinal polyene methylation affects receptor activation

Biochemistry 45:1640-1652   PubMed  URL





Lüdeke S, Beck M, Yan EC, Sakmar TP, Siebert F, Vogel R

The role of Glu 181 in the photoactivation of rhodopsin

J Mol Biol 353: 345-356   PubMed  URL


Vogel R, Lüdeke S, Siebert F, Hirshfeld A, Sheves M

Agonists and partial agonists of rhodopsin: retinals with ring modifications

Biochemistry 44: 11684-11699    PubMed  URL





Ruprecht J, Mielke T, Vogel R, Villa C, Schertler GFX 

Electron crystallography reveals the structure of Metarhodopsin I

EMBO J 23: 3609-3620     PubMed  URL


Vogel R, Lüdeke S, Radu I, Siebert F, Sheves M 

Photoreactions of Metarhodopsin III

Biochemistry 43: 10255-10264    PubMed   URL


Vogel R, Siebert F, Zhang XY, Fan GB, Sheves M

Formation of Meta III during the decay of activated rhodopsin proceeds via Meta I and not via Meta II

Biochemistry 43: 9457-9466   PubMed  URL


Vogel R

Influence of salts on rhodopsin photoproduct equilibria and protein stability

Curr Opin Colloid Interf Sci 9: 133-138    URL


Vogel R, Ruprecht J, Villa C, Mielke T, Schertler GFX, Siebert F

Rhodopsin photoproducts in 2D crystals

J Mol Biol 338:597-609   PubMed   URL





Vogel R, Siebert F, Mathias G, Tavan P, Fan GB, and Sheves M

Deactivation of rhodopsin in the transition from the signaling state Meta II to Meta III involves a thermal isomerization of the retinal chromophore C=N double bond

Biochemistry 42: 9863-9874    PubMed   URL


Vogel R, Siebert F

Fourier transform infrared spectroscopy study for new insights into molecular properties and activation mechanisms of visual pigment rhodopsin

Biospectroscopy 72: 133-148     PubMed URL





Fan GB, Siebert F, Sheves M, Vogel R

Rhodopsin with 11-cis-locked chromophore is capable of forming an active state photoproduct

J Biol Chem 277: 40229-40234    PubMed  URL


Vogel R, Fan GB, Lüdeke S, Siebert F, Sheves M 

A non-bleachable rhodopsin analogue with a slow photocycle

J Biol Chem 277: 40222-40228   PubMed   URL


Vogel R, Siebert F

Conformation and stability of alpha-helical membrane proteins: I. Influence of salts on conformational equilibria between active and inactive states of rhodopsin

Biochemistry 41: 3529-3535   PubMed  URL


Vogel R, Siebert F

Conformation and stability of alpha-helical membrane Proteins: II. Influence of pH and salts on stability and unfolding of rhodopsin

Biochemistry 41: 3536-3445   PubMed   URL





Vogel R, Siebert F

Conformations of the active and the inactive states of opsin

J Biol Chem 276: 38487-38493  PubMed   URL


Vogel R, Fan GB, Siebert F, Sheves M

Anions specifically stabilize a Metarhodopsin II-like photoproduct with a protonated Schiff base

Biochemistry 40: 13342-13352     PubMed  URL


Vogel R, Fan GB, Sheves M, Siebert F

Salt Dependence of the formation and stability of the signaling state in G protein-coupled receptors: Evidence for the involvement of the Hofmeister effect

Biochemistry 40:  483-493    PubMed   URL





Vogel R, Siebert F

Vibrational spectroscopy as a tool for probing protein function

Curr Opin Chem Biol 4: 518-523     PubMed  URL


Kiefer H, Vogel R, Maier K

Bacterial expression of G-protein coupled receptors: Prediction of expression levels from sequence

Receptors Channels 7: 109-119   PubMed  URL


Vogel R, Fan GB, Sheves M, Siebert F

The molecular origin of the inhibition of transducin activation in rhodopsin lacking the 9-methyl group of the retinal chromophore: A UV-vis and FTIR spectroscopic study

Biochemistry 39: 8895-8908     PubMed   URL





Kiefer H, Maier K, Vogel R

Refolding of G protein-coupled receptors from inclusion bodies produced in E.coli

Biochem Soc Trans 27: 908-912    PubMed


Vogel R, Süssmuth R

A model for the generation of low level chemiluminescence from microbiological growth media and its depletion by bacterial cells

Bioelectrochem Bioenerg 48: 375-383    PubMed  URL


Vogel R, Süssmuth R

Low level chemiluminescence from liquid culture media

J Appl Microbiol 86: 999-1007    PubMed  URL


Vogel R, Süssmuth R

Weak light emission patterns from lactic acid bacteria

Luminescence 14: 99-105   PubMed URL





Vogel R, Süssmuth R

Involvement of the cell membrane in chemiluminescence patterns from bacterial cultures

Bioelectrochem Bioenerg 46: 65-69    URL


Vogel R, Guo X, Süssmuth R

Chemiluminescence patterns from bacterial cultures undergoing bacteriophage-induced mass lysis

Bioelectrochem Bioenerg 46: 59-64    URL


Vogel R, Süssmuth R

Interaction of bacterial cells with weak light emission from culture media

Bioelectrochem Bioenerg 45: 93-101    URL




Sokolov IM, Vogel R, Alemany PA, Blumen A 

Continuous-time random walk of a rigid triangle

J Phys A 28: 6645-6653  URL



Alemany PA, Vogel R, Sokolov IM, Blumen A

A dumbbell's random walk in continuous time

J Phys A 27: 7733-7738  (1994)   URL